Fk2 antibody

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August undefined, 2024

WebImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-214). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-214) at 1:100 for 60 min at RT. WebMouse Monoclonal Antibody Shop MilliporeSigma™ anti-Ubiquitin Clone: FK2, at Fishersci.com. Fisher Scientific ; ... All Primary Antibodies ST1200100UG Print MilliporeSigma™ anti-Ubiquitin Clone: FK2, ...

Anti-Ubiquitin Antibody - Antibodies Against Ubiquitin - LifeSensors

WebApr 14, 2024 · Ubiquitinated proteins, detected by the monoclonal antibody FK2 (ub-FK2), and RNF8-dependent ubiquitinated H2A (ub-H2A, detected by monoclonal antibody E6C5) are localized on the sex body area (Lu et al., 2010). K48- and K63-linked poly-ubiquitins are enriched in the XY body (Lu et al., 2010). The role of ubiquitination around the XY body ... WebBlock: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody (SMC-550) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti … bravlee chair covers

Anti-Ubiquitin Mouse mAb (FK2) ST1200 - Merck Millipore

WebThe Tb-anti-ubiquitin-FK2 antibody preferentially binds to mono- or poly-ubiquitinated proteins over free ubiquitin. This antibody can be used in combination with purified GFP … Web(C) Ubiquitin IP with the FK2 antibody from the cells expressing the indicated proteins, followed by IB with FK2 and the pan-ubiquitin antibody #3933. (D) IP using the HA antibody from the cells co-expressing HA-ubiquitin with the indicated proteins, followed by IB with the HA and pan-ubiquitin antibodies. correo web sign in movistar

Anti-Ubiquitin Antibody [FK2] Rabbit Monoclonal

A versatile new tool derived from a bacterial deubiquitylase to …

WebImmunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ … WebUbiquitin Antibody (FK2) [Unconjugated] Summary. This antibody will recover mono- and polyubiquitinated proteins in I.P. applications. This antibody detects mono- and … correo web speedyWebThe FK2 antibody works great in both human and rat samples (I haven't tried mouse) by western blot and immunofluorescence. This antibody should recognize the ubiquitin modifications (both mono... bravo 119 flac warez

"WebSep 10, 2016 · Scheme of the ELISA assay. (a) In the “forward” approach, microwell plates are coated with a polyclonal anti-EGFR intra, which captures the receptor from the lysate (1).Detection of Ub-EGFR or total EGFR (to normalize) is performed with primary monoclonal antibodies directed against Ub (FK2, 1) or EGFR extra (2), respectively, … " - Fk2 antibody

Fk2 antibody

Bacterial secretion system skews the fate of - Nature

WebClone FK2 is the most widely used ubiquitin antibody. This clone reacts with mono- and polyubiquitinated conjugates, but does not react with free ubiquitin. 1, 2 WARNING This product is not for human or veterinary use. Certificates of Analysis & Batch Specific Data Get Batch-Specific Data and Documents by Batch Number WebJun 30, 2024 · For the analysis of human cells, OtUBD pulldowns performed better than FK2 antibody immunoprecipitations in our hands, both in terms of overall efficiency and repeatability. Inconsistency in the performance of antibodies from different batches has been a widely recognized issue . Recombinant OtUBD, by contrast, appeared to be …

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Web(B and C) Mono- and poly-ubiquitinated proteins detection using the FK2 specific monoclonal antibody (B) Quantification of ubiquitinated protein detection after normalization to Actin (C). (D) Control and treated RHEs were stained with haematoxylin–eosin (n = 2, 1 independent bank). Bar = 10 μm. WebSep 15, 2013 · The FK2 antibody is a mouse monoclonal recognizing both monoubiquitinated and polyubiquitinated conjugates. According to the manufacturer’s data sheet the FK2 antibody can recognize various types of ubiquitin chains; however, it is unknown if it has equal affinities for all seven ubiquitin chain linkages and for different …

WebApr 14, 2024 · Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) 单泛素/多泛素偶联物单抗（FK2）品牌：Enzo; 13HP020AN-东洋一次性针头式过滤器0.2um孔径; 月桂硫酸盐肉汤培养基-其它; Whatman 滤膜 6720-5001 盘式滤器 Polydisc TF, 0.1m 10/盒 WebCreative Biolabs offers high-quality Mouse Anti-Ubiquitin Monoclonal Antibody (FK2), Conjugated to boost neuroscience research. Toggle navigation. Service . In Vitro Services. Cell Culture Models; ... New Antibodies to Phospho Alpha Synuclein and Tau; Pre-made AAV Center; New Fast Genetically Encoded Calcium Indicators: jGCaMP8;

WebJan 7, 2024 · product name : Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) catalog : BML-PW8810-0100 quantity : 100 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK2 reactivity : southern flounder, Saccharomycetales, human, mouse, rat, fruit fly application : WebFeb 11, 2024 · Anti-Multi Ubiquitin mAb(Monoclonal, FK2 from MBL.Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono …

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WebJan 7, 2024 · antibody product name : Polyubiquitinylated conjugates monoclonal antibody (FK1) catalog : BML-PW8805-0500 quantity : 500 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK1 reactivity : spiny dogfish, human, mouse, rat, fruit fly application : western blot, ELISA, immunohistochemistry, immunocytochemistry, … correoweb tigre gob arWebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the … bravlin law firmWebProperties Biological Description Product Images Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Rabbit Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-550). Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 µg. bravks maytag washer not filling upWebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin … correo web telmexWebHigh Quality Antibodies Delivering Superior Results. Enzo Life Sciences offers thousands of antibodies against peptides, whole or partial proteins, and modified peptides or proteins. Backed by thousands of peer … correoweb tscWeb(BML-PW8810-0500) Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2), 500 µg - Enzo Life Sciences - CiteAb 35 citations – A mouse monoclonal … bravley second yokai jobWebMar 20, 2024 · Ubiquitin staining was performed using anti-ubiquitin (FK2) antibody. The blue (Hoescht 33342) but not red bacteria are L. dumoffii. Ubiquitin was commonly associated with LpCVs (arrows), but was ... bravo 127 w burnside ave bronx ny