Phospho- ser/thr pka substrate antibody
WebSep 1, 2005 · Phospho- (Ser/Thr) PKA substrate antibody was from Cell Signaling Technology (Catalog number 9621). Speract was from Peninsula Laboratories. Anti-bindin antibody was prepared as described ( Moy and Vacquier, 1979 ). All other reagents and chemicals were from Sigma. WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for …
Phospho- ser/thr pka substrate antibody
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WebPhospho-(Ser/Thr) PKA Substrate Antibody 可检测包含磷酸-丝氨酸/苏氨酸残基（在 -3 位点有精氨酸）的肽和蛋白。它是一种有用的工具，可用来识别 AGC 家族激酶的底物，包括 … WebIn addition, a phospho- (Ser/Thr) PKA substrate antibody detected bands on immunoblot analysis of trans-sialidase antibody precipitated proteins from parasite lysate and the …
WebFor antiphospho-PKA substrate (RRXS/T) (100G7E) antibody, a rabbit monoclonal antibody against pPKA substrate was used (commercially available from Cell Signaling). For … WebPhospho-PKA alpha (Ser338) Polyclonal Antibody detects endogenous levels of PKA alpha only when phosphorylated at Ser338. Target Information Protein kinase A (PKA, cAMP …
WebAnti-Phospho - (Ser/Thr) antibody Description Rabbit polyclonal to Phospho - (Ser/Thr) Host species Rabbit Tested applications Suitable for: ELISA, Dot blot more details Species … WebApr 13, 2024 · In particular, we saw increased phosphorylation of connexin-43 at Ser325/Thr326, and decreased levels of the phospho-peptide, Ser-Thr 320-345 (Figure 4A’; Supplementary Table S4). Notably, the phosphorylation of Ser325 on connexin-43, along with nearby residues Ser328 and Ser330, is mediated by casein kinase 1 and has been shown …
WebBuy pka antibodies from Santa Cruz Biotechnology, Inc. Monoclonal antibodies are available to most protein immunogens. ... PKA Phosphopeptide Substrate Antibody (15B12) sc-65491: mouse IgG 1: N/A: ELISA: NSR: NEW (1) ... Phospho-Specific PKA Antibodies PRODUCT NAME CATALOG # ISOTYPE EPITOPE APPLICATIONS Species Citations
WebSee all Phospho - (Ser/Thr) Phe primary antibodies Description Rabbit polyclonal to Phospho - (Ser/Thr) Phe Host species Rabbit Tested applications Suitable for: ELISA, IP, WB, ICC/IF more details Species reactivity Reacts with: Species independent Immunogen Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . little brother dollWebMay 20, 2024 · Epigallocatechin-3-gallate (EGCG) has widespread effects on adipocyte development. However, the molecular mechanisms of EGCG are not fully understood. We investigate the adipogenic differentiation of human-derived mesenchymal stem cells, including lipid deposition and changes in the expression and phosphorylation of key … little brother electric johnson barWebDec 15, 2009 · (C) Thr-90-phosphorylation status of Hsp90α in the CM and TCL. pT90-Hsp90α was detected using anti-phospho-(Ser/Thr) PKA substrate antibody. (D) Secretion and Thr-90-phosphorylation status of Hsp90α upon overexpression of Flag-tagged PKA (Left) or upon siRNA treatment (Right) detected by Western blotting and ELISA. PKA was … little brother doctorowWebImmunogen was a synthetic phospho-PKA substrate peptide. Phospho- (Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine … little brother endingWebNov 2, 2024 · Proteins retained on sepharose were incubated with an active recombinant PKA Cα subunit for the kinase assay and then blotted with an anti-phospho- (Ser/Thr) PKA substrate antibody or anti-myc... little brother films gmbh betrugWebThe NeuroMab™ anti-Phospho-PKA C (Thr197) antibody detects endogenous levels of PKA C (-α, -β, and -γ) only when phosphorylated at Thr197. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr197 of human PKA C protein. Store at -20°C. Do not aliquot the antibody. little brother doxWebImmunodetection of phospho- (Ser/Thr) PKA substrates shows that phosphorylated proteins are localized differently in spermatozoa of carp and sterlet. Strong phosphorylation of PKC substrate was observed in flagella of sterlet spermatozoa, whereas in carp sperm, PKC substrates were lightly phosphorylated in the midpiece and flagella. little brother death poems