Promega lysis buffer
WebSep 4, 2014 · After 48 h of transfection, cells were harvested and lysed in 200 μL of reporter lysis buffer (Promega, Madison, WI, USA). A luciferase assay was carried out using a dual luciferase assay kit (Promega), and the enzymatic activity of luciferase was measured using a luminometer (Promega). WebThe efficiencyof lysis may depend on growth conditions and cell or tissue type. Lysis Buffer Compatible Reporter Assay Protein Assay(4) Western Blot(5) Cell Culture Lysis Reagent …
Promega lysis buffer
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WebThe Reporter Lysis Buffer allows β-gal and luciferase assays to be performed on cell lysates of cells cotransfected with vectors carrying these reporter genes. In cell lines tested at Promega, β-gal activity in cells lysed with Reporter Lysis Buffer is significantly higher than activity in lysates prepared by the freeze-thaw method. Specifications Web¥ 14,000 Glo Lysis Buffer (GLB)は強力で細胞の掻取りや凍結/融解操作が不要です。 バッファーを添加するだけで、通常5分以内に細胞を溶解することができます。 特にグロータ …
WebPassive Lysis 5X Buffer 30ml ¥ 14,000 Passive Lysis Buffer, 5X, is specifically formulated to promote rapid lysis of cultured mammalian cells without the need for scraping adherent … WebConvenient and Easy to Use. Simply mix buffer with lyophilized substrate and add to cells in culture medium; no need to thaw or measure before use. Learn more about custom options for this product at: www.promega.com/custom/ Contact Promega for …
WebPromega Nano-Glo™ Luciferase Assay System The Nano-Glo™ Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media. $286.00 - $12150.00 Specifications View More Specs Products 4 WebApr 15, 2024 · The secondary goat anti-mouse horseradish peroxidase (HRP)-conjugated IgG antibody (75 µL; 1:5000 in 5% albumin—Promega, Southampton, UK) was added, and the plate was incubated for 1 h at RT in the dark. ... 1X PBS or lysis buffer was added to the coated/blocked-well, where high signal was recorded due to the detection of CoA on the …
WebMy colleagues and I have been struggling to find a sufficient cell lysis buffer for our luciferase assay. We have been using luciferase, luciferin, varying detergents (SDS, Tween 20, Triton)...
WebRNA Lysis Buffer (RLA) is used to lyse cells during RNA purification Component of Promega RNA purification kits available for purchase separately Size 50ml Catalog number … pruftechnik alignment toolsWebSep 14, 2024 · PCR inhibitors—from both the sample and the lysis buffer utilized to lyse and release the RNA—have the potential to skew quantification in qPCR. In a typical PCR workflow, the solution to PCR inhibition is to purify or dilute the RNA extracts, which leads to a … prufund bonus ratesWebFeb 27, 2015 · of course you can use ready to use lysis buffer (RIPA) from many companies too. some papers used: PBS, pH 7.4, SDS, 0.1%, Na-deoxycholate, 0.25% best mehdi Cite 27th Feb, 2015 Mingcai Zhang... pruftechnik vibration monitoringWebLysis Buffer is designed to work with the other components of the DNA IQ™ System (Cat.# DC6700 and DC6701) to purify DNA from blood, blood stains, buccal swabs and a variety … resume objective for production supervisorWebRNA Lysis Buffer (RLA) is used to lyse cells during RNA purification Component of Promega RNA purification kits available for purchase separately Size 50ml Catalog number selected: Z3051 € 47,00 Your price: Log in Add to Cart Add to Helix RNA Lysis Buffer (RLA) 50ml € 47,00 Your price: Log in prufund announcementWebApr 6, 2024 · The global Luciferase Assay Lysis Buffer market was valued at USD million in 2024 and is anticipated to reach USD million by 2029, witnessing a CAGR during the forecast period 2024-2029. The... prufund additional investment formWebLysis Buffer A8261 PDF (230 KB) – English (United States ... Stay notified of Promega events, products and news. Sign Up. Need Assistance? Contact Promega; Customer and … resume objective for scholarship example